Journal: International Journal of Molecular Sciences

Article Title: Tiliroside Protects against Lipopolysaccharide-Induced Acute Kidney Injury via Intrarenal Renin–Angiotensin System in Mice

doi: 10.3390/ijms242115556

Figure Lengend Snippet: Molecular docking poses of Tiliroside with renin (PDB ID 4RYC) ( A ), ACE (PDB ID 6TT1) ( B ), and ACE2 (PDB ID 7V78) ( C ), aliskiren and renin ( D ), enalapril and ACE ( E ), MLN-4760 and ACE2 ( F ). ( G ) The binding affinity between Tiliroside and renin, ACE, and ACE, aliskiren and renin, enalapril and ACE, MLN-4760, and ACE2.

Article Snippet: Total renin/prorenin, Ang II, Ang1-7, albumin, NGAL, and KIM-1 released into the urine were measured by ELISA assay using a total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, MI, USA), Ang II ELISA kit (ADI-900-204, Enzo Life Sciences, Farmingdale, NY, USA), mouse Ang1-7 ELISA kit (EM1634, Wuhan Fine Biotech Co., Ltd., Wuhan, China), mouse NGAL ELISA KIT (SEKM-0119, Solarbio, Beijing, China), and mouse KIM-1 ELISA KIT (SEKM-0147, Solarbio, Beijing, China), respectively.

Techniques: Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Tiliroside Protects against Lipopolysaccharide-Induced Acute Kidney Injury via Intrarenal Renin–Angiotensin System in Mice

doi: 10.3390/ijms242115556

Figure Lengend Snippet: Effect of Tiliroside on renal ACE and ACE activity, urinary renin, Ang II, and Ang1-7 excretion, and plasma Ang II and Ang1-7 concentration in LPS-induced AKI mice. ( A ) Renal ACE activity. ( B ) Urinary renin activity. ( C ) 24 h urinary prorenin/renin excretion. ( D ) 24 h urinary Ang II excretion. ( E ) Renal ACE2 activity. ( F ) 24 h urinary Ang1-7 excretion. ( G ) Plasma Ang II concentration. ( H ) Plasma Ang1-7 concentration. n = 5–8 in each group. ** p < 0.01 and *** p < 0.001 vs. Vehicle; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. LPS.

Article Snippet: Total renin/prorenin, Ang II, Ang1-7, albumin, NGAL, and KIM-1 released into the urine were measured by ELISA assay using a total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, MI, USA), Ang II ELISA kit (ADI-900-204, Enzo Life Sciences, Farmingdale, NY, USA), mouse Ang1-7 ELISA kit (EM1634, Wuhan Fine Biotech Co., Ltd., Wuhan, China), mouse NGAL ELISA KIT (SEKM-0119, Solarbio, Beijing, China), and mouse KIM-1 ELISA KIT (SEKM-0147, Solarbio, Beijing, China), respectively.

Techniques: Activity Assay, Concentration Assay

Journal: American Journal of Physiology - Renal Physiology

Article Title: (Pro)renin receptor contributes to pregnancy-induced sodium-water retention in rats via activation of intrarenal RAAS and α-ENaC

doi: 10.1152/ajprenal.00411.2018

Figure Lengend Snippet: Renal (pro)renin receptor (PRR) expression is increased during normal late pregnancy. A: representative immunoblotting and densitometric analysis of full-length (fPRR) and soluble (sPRR) PRR protein in the renal cortex and renal inner medulla. The densitometry values are shown underneath the corresponding blots. Expression was normalized by β-actin; n = 8–12/group. B: ELISA measurement of urinary sPRR excretion; n = 8–12/group. C: representative immunofluorescence images of cortical and inner medullary PRR in control (CTR) and Pregnancy group rats. D: statistical results of semiquantitative analysis for the relative fluorescence intensity of all immunofluorescence images; n = 6/group. Values are means ± SE. **P < 0.01 vs. CTR.

Article Snippet: Total renin/prorenin, sPRR, angiotensin II, and aldosterone levels, released into the plasma and urine, were measured by using a total rat renin/prorenin ELISA kit (RPRENKT-TOT, Molecular Innovations, Novi); sPRR assay kit (cat. no. 27782; Immuno-Biological Laboratories, Gunma, Japan); angiotensin II ELISA kit (ADI-900–204; Enzo Life Sciences, Farmingdale, NY) and aldosterone ELISA kit (cat. no. 10004377; Cayman Chemical, Ann Arbor, MI), according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Fluorescence

Journal: American Journal of Physiology - Renal Physiology

Article Title: (Pro)renin receptor contributes to pregnancy-induced sodium-water retention in rats via activation of intrarenal RAAS and α-ENaC

doi: 10.1152/ajprenal.00411.2018

Figure Lengend Snippet: Renal PRR primarily increases intrarenal renin activity but not renin expression during normal late pregnancy. The urine samples were assayed for urinary renin activity (A), urinary active renin content (B), urinary prorenin content (C), and urinary total renin content (D). The urine samples (E) and plasma samples (F) were assayed for total renin/prorenin by ELISA. Of note, the ELISA kit was unable to differentiate between prorenin and renin. qRT-PCR was performed to analyze renin mRNA expression in the renal cortex (G) and renal inner medulla (H); n = 8–to 12/group. **P < 0.01 vs. CTR. #P < 0.05 and ##P < 0.01 vs. Pregnancy.

Article Snippet: Total renin/prorenin, sPRR, angiotensin II, and aldosterone levels, released into the plasma and urine, were measured by using a total rat renin/prorenin ELISA kit (RPRENKT-TOT, Molecular Innovations, Novi); sPRR assay kit (cat. no. 27782; Immuno-Biological Laboratories, Gunma, Japan); angiotensin II ELISA kit (ADI-900–204; Enzo Life Sciences, Farmingdale, NY) and aldosterone ELISA kit (cat. no. 10004377; Cayman Chemical, Ann Arbor, MI), according to the manufacturer’s instructions.

Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR